[Superseded]

separate() has been superseded in favour of separate_wider_position() and separate_wider_delim() because the two functions make the two uses more obvious, the API is more polished, and the handling of problems is better. Superseded functions will not go away, but will only receive critical bug fixes.

Given either a regular expression or a vector of character positions, separate() turns a single character column into multiple columns.

# S3 method for class 'SpatialExperiment'
separate(
  data,
  col,
  into,
  sep = "[^[:alnum:]]+",
  remove = TRUE,
  convert = FALSE,
  extra = "warn",
  fill = "warn",
  ...
)

Arguments

data

A data frame.

col

<tidy-select> Column to expand.

into

Names of new variables to create as character vector. Use NA to omit the variable in the output.

sep

Separator between columns.

If character, sep is interpreted as a regular expression. The default value is a regular expression that matches any sequence of non-alphanumeric values.

If numeric, sep is interpreted as character positions to split at. Positive values start at 1 at the far-left of the string; negative value start at -1 at the far-right of the string. The length of sep should be one less than into.

remove

If TRUE, remove input column from output data frame.

convert

If TRUE, will run type.convert() with as.is = TRUE on new columns. This is useful if the component columns are integer, numeric or logical.

NB: this will cause string "NA"s to be converted to NAs.

extra

If sep is a character vector, this controls what happens when there are too many pieces. There are three valid options:

  • "warn" (the default): emit a warning and drop extra values.

  • "drop": drop any extra values without a warning.

  • "merge": only splits at most length(into) times

fill

If sep is a character vector, this controls what happens when there are not enough pieces. There are three valid options:

  • "warn" (the default): emit a warning and fill from the right

  • "right": fill with missing values on the right

  • "left": fill with missing values on the left

...

Additional arguments passed on to methods.

Value

tidySpatialExperiment

See also

unite(), the complement, extract() which uses regular expression capturing groups.

Examples

example(read10xVisium)
#> 
#> rd10xV> dir <- system.file(
#> rd10xV+   file.path("extdata", "10xVisium"), 
#> rd10xV+   package = "SpatialExperiment")
#> 
#> rd10xV> sample_ids <- c("section1", "section2")
#> 
#> rd10xV> samples <- file.path(dir, sample_ids, "outs")
#> 
#> rd10xV> list.files(samples[1])
#> [1] "raw_feature_bc_matrix" "spatial"              
#> 
#> rd10xV> list.files(file.path(samples[1], "spatial"))
#> [1] "scalefactors_json.json"    "tissue_lowres_image.png"  
#> [3] "tissue_positions_list.csv"
#> 
#> rd10xV> file.path(samples[1], "raw_feature_bc_matrix")
#> [1] "/__w/_temp/Library/SpatialExperiment/extdata/10xVisium/section1/outs/raw_feature_bc_matrix"
#> 
#> rd10xV> (spe <- read10xVisium(samples, sample_ids, 
#> rd10xV+   type = "sparse", data = "raw", 
#> rd10xV+   images = "lowres", load = FALSE))
#> # A SpatialExperiment-tibble abstraction: 99 × 7
#> # Features = 50 | Cells = 99 | Assays = counts
#>    .cell              in_tissue array_row array_col sample_id pxl_col_in_fullres
#>    <chr>              <lgl>         <int>     <int> <chr>                  <int>
#>  1 AAACAACGAATAGTTC-1 FALSE             0        16 section1                2312
#>  2 AAACAAGTATCTCCCA-1 TRUE             50       102 section1                8230
#>  3 AAACAATCTACTAGCA-1 TRUE              3        43 section1                4170
#>  4 AAACACCAATAACTGC-1 TRUE             59        19 section1                2519
#>  5 AAACAGAGCGACTCCT-1 TRUE             14        94 section1                7679
#>  6 AAACAGCTTTCAGAAG-1 FALSE            43         9 section1                1831
#>  7 AAACAGGGTCTATATT-1 FALSE            47        13 section1                2106
#>  8 AAACAGTGTTCCTGGG-1 FALSE            73        43 section1                4170
#>  9 AAACATGGTGAGAGGA-1 FALSE            62         0 section1                1212
#> 10 AAACATTTCCCGGATT-1 FALSE            61        97 section1                7886
#> # ℹ 89 more rows
#> # ℹ 1 more variable: pxl_row_in_fullres <int>
#> 
#> rd10xV> # base directory 'outs/' from Space Ranger can also be omitted
#> rd10xV> samples2 <- file.path(dir, sample_ids)
#> 
#> rd10xV> (spe2 <- read10xVisium(samples2, sample_ids, 
#> rd10xV+   type = "sparse", data = "raw", 
#> rd10xV+   images = "lowres", load = FALSE))
#> # A SpatialExperiment-tibble abstraction: 99 × 7
#> # Features = 50 | Cells = 99 | Assays = counts
#>    .cell              in_tissue array_row array_col sample_id pxl_col_in_fullres
#>    <chr>              <lgl>         <int>     <int> <chr>                  <int>
#>  1 AAACAACGAATAGTTC-1 FALSE             0        16 section1                2312
#>  2 AAACAAGTATCTCCCA-1 TRUE             50       102 section1                8230
#>  3 AAACAATCTACTAGCA-1 TRUE              3        43 section1                4170
#>  4 AAACACCAATAACTGC-1 TRUE             59        19 section1                2519
#>  5 AAACAGAGCGACTCCT-1 TRUE             14        94 section1                7679
#>  6 AAACAGCTTTCAGAAG-1 FALSE            43         9 section1                1831
#>  7 AAACAGGGTCTATATT-1 FALSE            47        13 section1                2106
#>  8 AAACAGTGTTCCTGGG-1 FALSE            73        43 section1                4170
#>  9 AAACATGGTGAGAGGA-1 FALSE            62         0 section1                1212
#> 10 AAACATTTCCCGGATT-1 FALSE            61        97 section1                7886
#> # ℹ 89 more rows
#> # ℹ 1 more variable: pxl_row_in_fullres <int>
#> 
#> rd10xV> # tabulate number of spots mapped to tissue
#> rd10xV> cd <- colData(spe)
#> 
#> rd10xV> table(
#> rd10xV+   in_tissue = cd$in_tissue, 
#> rd10xV+   sample_id = cd$sample_id)
#>          sample_id
#> in_tissue section1 section2
#>     FALSE       28       27
#>     TRUE        22       22
#> 
#> rd10xV> # view available images
#> rd10xV> imgData(spe)
#> DataFrame with 2 rows and 4 columns
#>     sample_id    image_id   data scaleFactor
#>   <character> <character> <list>   <numeric>
#> 1    section1      lowres   ####   0.0510334
#> 2    section2      lowres   ####   0.0510334
spe |>
    separate(col = sample_id, into = c("A", "B"), sep = "[[:alnum:]]n")
#> # A SpatialExperiment-tibble abstraction: 99 × 9
#> # Features = 50 | Cells = 99 | Assays = counts
#>    .cell  in_tissue array_row array_col A     B     sample_id pxl_col_in_fullres
#>    <chr>  <lgl>         <int>     <int> <chr> <chr> <chr>                  <int>
#>  1 AAACA… FALSE             0        16 secti 1     section1                2312
#>  2 AAACA… TRUE             50       102 secti 1     section1                8230
#>  3 AAACA… TRUE              3        43 secti 1     section1                4170
#>  4 AAACA… TRUE             59        19 secti 1     section1                2519
#>  5 AAACA… TRUE             14        94 secti 1     section1                7679
#>  6 AAACA… FALSE            43         9 secti 1     section1                1831
#>  7 AAACA… FALSE            47        13 secti 1     section1                2106
#>  8 AAACA… FALSE            73        43 secti 1     section1                4170
#>  9 AAACA… FALSE            62         0 secti 1     section1                1212
#> 10 AAACA… FALSE            61        97 secti 1     section1                7886
#> # ℹ 89 more rows
#> # ℹ 1 more variable: pxl_row_in_fullres <int>